PGTA: Preimplantation Genetic Diagnosis/Testing in HSR Layout, Bangalore
PGT-A ( formerly known as PGS ) is a genetic test performed on the embryos to identify numerical chromosomal abnormalities ( aneuploidy). This is a genetic study of the embryo produced during IVF treatment and can help you have a healthy baby.
Who should use PGT-A?
1. Advanced maternal age : >35yrs old and above.
2. Recurrent miscarriages: couples who had 2 or more miscarriages.
3. IVF failures : 2 or more ivf failures
4. Male factor infertility : low quantity and/or quality of sperm
Steps of PGT-A procedure :
1. Evaluation of couple
2. Stimulation of ovaries and collection of eggs
3. Fertilization with ICSI
4. Culture of the embryos to Blastocyst stage ( day 5 - 6)
5. Few cells are microscopically removed (biopsied) from the embryo - trophectoderm cell biopsy. These cells are sent to genetic laboratory for PGT testing.
6. After biopsy, embryos are immediately frozen and stored.
7. Once the results are ready , embryos which are abnormal are discarded and normal good embryos are used for FROZEN EMBRYO TRANSFER CYCLE.
Advantages of PGT-A:
1. Reduced miscarriage rates
2. Higher pregnancy rates per transfer
3. Fewer cycles of IVF treatment needed- reducing both time and financial cost.
4. Greater chance of having a healthy child.
5. Decreases chances of IVF failure due to implantation failure.
6. Decreases chances of IVF failure with aneuploid embryos that would never lead to a successful pregnancy.
7. Gives option of single embryo transfer ( SET)
8. Single embryo transfer (SET) helps in reducing the likelihood of multiple pregnancies and avoids its complications.
FAQ's on PGT-A Treatment
The three different PGTs are all carried out similarly. The patient follows the doctor's advice and has an IVF cycle and egg retrieval. Their embryos are observed in our lab till they are referred to as blastocysts on day 5 or day 6. A few cells are extracted from the biopsyed (removed) embryos and sent to a lab for PGT. The blastocyst's trophectoderm eventually develops into the placenta.
It is anticipated that these cells will constitute the remainder of the embryo, although due to things like mosaicism, this may not always be the case (see question 13). The embryo(s) must be frozen before PGT. An embryo with normal PGT results will be chosen, frozen, and implanted into the patient's uterus later.
You should decide to have PGT a long time before your IVF cycle. Before an IVF cycle can occur, DNA samples from the patient, their partner or donor, and occasionally other family members, may need to be obtained.
To see if the lab will be able to accurately identify the genetic alterations in the patient's embryo, PGT-M is put through a test development phase. The test creation stage can take up to 12 weeks after receiving all DNA samples. This procedure must be finished in its entirety before an IVF cycle (stimulating drugs) can begin.
The main goal of a PGT-A embryo is to guarantee that it is euploid, or having the appropriate amount of chromosomes. PGT-A can identify monosomies (incomplete chromosomes) and trisomies (incomplete chromosomes) in embryos. PGT-A also checks for chromosomal duplications and deletions. Large deletions and duplications are necessary for their detection. In some versions, but not all, the PGT-A may also pick up on mosaicism and uniparental disomy (UPD). Our genetic counsellor will go over the different kinds of outcomes that can be discovered during your session.
info@vrikshfertility.com
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